DNA Molecular Weight Marker II, DIG-labeled

Description

• General description: DNA Molecular Weight Marker II, DIG-labeled is a solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. This fragment mixture is prepared by cleavage of λDNA with Hind III. The size of the fragment ranges from 0.12 to 23.1 kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.
• Application: DNA Molecular Weight Marker II, DIG-labeled has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.[1][2]
• Features and Benefits: ContentsReady-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.
• Sequence: The mixture contains 8 fragments with the following base pair lengths: 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130 bp.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
• Other Notes: For life science research only. Not for use in diagnostic procedures.