GenElute™ HP Plasmid Maxiprep Kit

Description

• General description: The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used. An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
• Application: GenElute™ HP Plasmid Maxiprep Kit has been used in plasmid DNA extraction of SF91 or MIEV vector[1], apolipoprotein A-V mutant constructs[2], lentiviral vector pLenti6/R4R2/V5-DEST[3],metastasis-associated protein 1 (MTA1)[4] and adipokinetic hormone receptor[5]
• Features and Benefits: From harvested bacterial culture to pure plasmid DNA in 30 minutes or lessUp to 1.2 mg of high-copy plasmid DNAFlexibility of a vacuum or spin formatContains fewer plastic components than other high speed kits, reducing the amount of wasteNo phenol/chloroform extraction or alcohol precipitation required
• Other Notes: For additional information, please see www.sigma-aldrich.com/genelutehp.
• Legal Information: GenElute is a trademark of Sigma-Aldrich Co. LLC