Pectolyase from Aspergillus japonicus

Description

• General description: We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
• Application: Pectolyase from Aspergillus japonicus has been used:in the lysis of chlorella cells for protoplast generation[2] as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation[3]to induce reorganization of Arabidopsis root cap cell architecture[4]
• Biochem/physiol Actions: Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
• Other Notes: Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
• Unit Definition: One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.