Description

• General description: We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
• Application: Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene[1].
• Biochem/physiol Actions: Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
• Unit Definition: One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.
• Physical form: Contains (NH4)2SO4 and sodium chloride
• Analysis Note: Protein determined by biuret.
• Other Notes: View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer