Spectrum™ Plant Total RNA Kit

Description

• General description: The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg. After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants. Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
• Application: Spectrum®Plant Total RNA Kit has been used:to extract RNA from the material ground in liquid nitrogen[1]to isolate total RNA from mutant and wt (wild type) plantlets[2]to extract total RNA from leaf samples[3]to extract RNA from all plant tissues[4]
• Features and Benefits: Yields up to 60 μg of pure concentrated RNA per prepEfficient protocol allows for RNA purification in 30 min or lessSpecially designed for research with difficult plant tissues
• Legal Information: Specac is a registered trademark of Specac Ltd.