Description

• General description: Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.
• Application: Fractionation Range (MW)Dextrans: 100 - 5,000Globular Proteins 1,000 - 5,000Sephadex® G-25 has been used: in the desalting of protease[1]in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation[2]
• Biochem/physiol Actions: Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.
• Features and Benefits: Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.High recovery rate with minimal sample dilution.Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.Specifically designed as a BioProcess resin for industrial applications.
• Legal Information: Sephadex is a registered trademark of Cytiva