11-102-1850

TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 11-102-1850-Each-of-1 In Stock ₹ 2,14,579.00

11-102-1850 - Each of 1

₹ 2,14,579.00

In Stock

Quantity

1

Base Price: ₹ 2,14,579.00

GST (18%): ₹ 38,624.22

Total Price: ₹ 2,53,203.22

Diameter (Metric)

5 mm

Length (Metric)

5 cm

Particle Size

10 μm

Solvents

≤ 20% Organic Solvent

Stationary Phase

M-Aminophenyl Boronate

Sample Type

Proteins

Material

Glass

Flow Rate

1.2 mL/min

Max. Pressure

30 bar

pH

2 to 9

Temperature

>0°C

Product Line

TSKgel

Matrix

M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer

Technique

Affinity

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Description

  • High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds, and catecholamines Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers Adsorption onto the matrix takes place in basic buffers such as HEPES and morpholine Desorption takes place in carbohydrate or amine-containing mobile phases such as sorbitol or Tris Resin Adsorption Capacity: 40 μmol/mL