Thermo Scientific™ CaptureSelect™ CH1-XL MiniChrom™ Pre-packed Column

Description

• CaptureSelect CH1-XL MiniChrom™ columns are designed for bench-scale resin screening, process development, and sample preparation
• These pre-packed columns are ready to connect and have the following features: Dimensions of 1 mL (0.8 x 2 cm) and 5 mL (0.8 x 10 cm) Direct connection to standard chromatography systems Packed with CaptureSelect CH1-XL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates The 1 mL columns can be used for quick screenings of application feasibility and lab scale purification on a convenient and easy-to-use pre-packed column
• The 10 cm bed height of the 5 mL columns allows initial process development on a bench scale
• The CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers
• In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4)
• Features of this affinity matrix include: Platform approach for purification of high quality and correctly assembled human Fab fragments No co-purification of over-expressed free light chains due to selectivity for the CH1 domain Elution possible at pH 4.0–4.5 with 50 mM acetate buffer Caustic cleaning with up to 50 mM NaOH Excellent scalability No cross binding to bovine IgG, therefore presence of fetal bovine serum is not a problem Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics: Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect CH1-XL affinity ligand Ligand coupling method: epoxide coupling Binding capacity: >15 mg/mL human Fab fragment, depending on flow rate, column height, and contact time
• Note that humanized Fab fragments may have lower capacities
• Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0 - 4.5 Column dimensions: 1 mL (0.8 x 2 cm) and 5 mL (0.8 x 10 cm) Flow characteristics: 75–150 cm/h (up to 2 bar) Formulation buffer: 20%(v/v) ethanol