4591510

Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Manufacturer: Fischer Scientific

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For Use With (Application)

For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales

Storage Requirements

2°C to 8°C

Flow Rate

150 to 250 cm/Hoμr

Height (English) Bed

9.84 in.

Max. Pressure

100 kPa

pH Range

2 to 9

Ligand Type

Recombinant Protein A (E. coli)

Stability

Stable in aqueous Buffers commonly used in Protein A Chromatography

Product Type

rProtein A Sepharose Fast Flow Media

Particle Size

60 to 165 μm

Height (Metric) Bed

25 cm

Matrix

4% Cross-Linked Agarose

Storage Buffer

20% Ethanol

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Description

  • Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage No mammalian components involved during manufacturing process Matrix: 4% cross-linked agarose Average particle size: 90μm (d50V) Ligand: 6mg rProtein A/mL medium ( E
  • coli ) Binding capacity: ∽50mg human IgG/mL media pH stability working range: 3 to 10 Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed

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For Use With (Application):
For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales

Storage Requirements:
2°C to 8°C

Flow Rate:
150 to 250 cm/Hoμr

Height (English) Bed:
9.84 in.

Max. Pressure:
100 kPa

pH Range:
2 to 9

Ligand Type:
Recombinant Protein A (E. coli)

Stability:
Stable in aqueous Buffers commonly used in Protein A Chromatography

Product Type:
rProtein A Sepharose Fast Flow Media

Particle Size:
60 to 165 μm

Height (Metric) Bed:
25 cm

Matrix:
4% Cross-Linked Agarose

Storage Buffer:
20% Ethanol

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