Thermo Scientific™ POROS™ HQ 50 μm Strong Anion Exchange Resin

Description

• POROS™ 50-micron resins are available for low pressure applications, including large scale downstream bioprocessing
• POROS HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography™ resin designed for the separation and purification of biomolecules
• Higher productivity—high throughput and high dynamic capacity associated with Perfusion Chromatography Chemical stability—allows aggressive cleaning and sanitization Enhanced biomolecule access—provided via large pores, ranging between 500-10000 Ã… Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix Develop better separations methods in a shorter time frame—the speed of Perfusion Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation Reduce time-consuming sample prep You can replace many sample preparation steps with high-speed Perfusion Chromatography technology
• For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample
• Elution in a small volume of new buffer accomplishes both buffer exchange and concentration
• Create novel assays for more efficient analysis Perfusion Chromatography technology is not limited to standard modes of separation
• Both enzymes and affinity ligands can be immobilized on POROS media
• By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential
• High capacity, high resolution, high speed In contrast to conventional chromatography media, POROS Perfusion Chromatography resin particles are engineered to have two discreet classes of pores
• Large “throughpores” allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short “diffusive” pores inside
• By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced
• Diffusion is no longer limiting and flow rates can be dramatically increased—without compromising resolution or capacity
• Separations can be achieved at 1,000 to 5,000 cm/hr compared to 50 to 360 cm/hr for conventional resins