UbiCREST (Ubiquitin Chain Restriction Analysis) is designed to analyze the ubiquitin linkages present in free polyubiquitin chains or polyubiquitin chains conjugated to a substrate protein
Developed in the Komander lab, the UbiCREST approach utilizes the linkage specificity of the ovarian tumor domain (OTU) class of deubiquitinases (DUBs) to characterize the linkages present in a sample containing polyubiquitin
This kit provides DUBs that are useful for in vitro analysis of user supplied polyubiquitinated protein samples
The samples may be generated by in vitro ubiquitination reactions, or obtained from cell lysates or other complex mixtures following immunoprecipitation or other means of purification.