MilliporeSigma™ Upstate In Vivo Shape Reagent (Live Cell RNA Structure Analysis)

Description

• High cell-permeability for rapid modification of RNA Reacts with all RNA nucleotides for high resolution mapping Contains highly pure form of 2-methylnicotinic acid imidazolide (NAI) Forms 2'-O-adducts on single stranded regions of RNA that can be detected by Selective 2'-Hydroxyl Acylation Primer Extension (SHAPE) NAI rapidly absorbed into cells, formulated to have low levels of toxicity for use with live cells Reacts in unbiased fashion with all four RNA nucleotides Combination of low toxicity, rapid uptake lack of nucleotide bias enables high resolution RNA structure analysis in variety of living systems Packaging: Vial Color: Clear, Colourless Recommended Storage Store NAI at −20°C in a non-frost-ree freezer protected from light