Invitrogen™ GeneArt™ CRISPR Nuclease mRNA

Description

• GeneArt CRISPR Nuclease mRNA is formulated for superior quality and improved product performance
• The mRNA format is ready to transfect and eliminates time-consuming cloning required when using CRISPR vector systems
• This wild-type Cas9 mRNA has two nuclear localization signals (NLSs) which has been shown to be more efficient at targeting the Cas9 protein to the nucleus (1)
• The mRNA format is ready to transfect and eliminates time-consuming cloning required when using CRISPR vector systems
• Cas9 mRNA is co-transfected with a target-specific guide RNA (gRNA) to direct the Cas9 protein to the intended genome locus to create a double-stranded break
• The complete RNA format is a smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency
• Additionally, the Cas9 mRNA can be used in multiplex approaches with more than one gRNA
• Use this approach to determine which gRNA sequence works best for a particular target or to edit multiple genomic loci with one transfection
• Use our GeneArt Precision gRNA Synthesis Kit or GeneArt CRISPR Strings to make your target specific gRNA
• GeneArt CRISPR Nuclease Vector product features include: Fewer steps with no annealing, cloning, or vector required for screening experiments Smaller payload for improved delivery and better genome editing efficiency T7 promoter option to improve cleavage in difficult to transfect cells Easier for multiplexed gene editing Easier delivery by microinjection For research use only
• Not for use in diagnostic procedures
• Certifications CoA Recommended Storage Store in ultra-cold freezer (-68 to -85°C)
• Product may be stored short-term in freezer (-5 to -30°C).