Thermo Scientific™ IMAGEN™ Parainfluenza Virus Typing Kit using Direct Immunofluorescence Assay

Description

• Rapidly detect and differentiate Parainfluenza virus types 1, 2 and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations with Thermo Scientific™ IMAGEN™ Parainfluenza Virus Typing Kit
• This direct immunofluorescence assay utilizes type-specific monoclonal antibodies to detect and identify Parainfluenza virus types 1, 2 and 3 Parainfluenza viruses are members of the genus Paramyxovirus classified within the family Paramyxoviridae
• 1 There are 6 species of Paramyxovirus known to infect humans which include Parainfluenza viruses 1, 2, 3, and 4 (subtypes 4a and 4b), Mumps virus, and Newcastle Disease virus
• Of the 4 Parainfluenza viruses, types 1, 2, and 3 are now recognized as a major cause of acute respiratory disease in infants and children
• 2,3 Use IMAGEN Parainfluenza Kit as a rapid, sensitive, and specific method for detection and differentiation of Parainfluenza virus types 1, 2, and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations
• Rapid results—within a little over 30 minutes of receiving test sample or culture Simple—ready-to-use reagents plus all IMAGEN assays follow the same basic methodology Excellent sensitivity and specificity—high quality, well-characterized antibodies used in the assay provide excellent sensitivity and specificity Easy-to-read results Long shelf life—24 months The IMAGEN Parainfluenza Kit contains fluorescein isothiocyanate (FITC) conjugated monoclonal antibodies which bind specifically to either Parainfluenza virus types 1, 2 or 3
• Specimens are incubated with the FITC conjugated reagents for 15 minutes
• Excess reagent is then removed by washing with phosphate-buffered saline (PBS)
• The stained areas are mounted and viewed using epifluorescence illumination
• If Parainfluenza virus types 1, 2, or 3 are present then characteristic apple-green intracellular fluorescence is seen within infected cells, which contrasts with the red background staining of uninfected cells
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