Invitrogen™ Permeabilization Medium (Medium B)

Description

• Permeabilization Medium (Medium B) is a component of the FIX & PERM™ Cell Permeabilization Kit
• The kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics
• The FIX & PERM™ Cell Permeabilization Kit is: Flexible—compatible with multiple sample types and cellular antigens Robust—scatter pattern of cells unaffected Efficient—simultaneous detection of intracellular and surface markers View a selection guide for all available FIX & PERM™ products and products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information about the use of Dynabeads™ cell separation products for cell isolation)
• The Permeabilization Medium formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step
• How It Works Cells are incubated for 15 minutes at room temperature with Fixation Medium (Medium A) (Cat
• No
• GAS001S100) and washed
• Cells are then incubated with the Permeabilization Medium and fluorochrome-labeled antibody
• Both Fixation Medium and Permeabilization Medium are included in the FIX & PERM™ Cell Permeabilization Kit.