Thermo Scientific™ PHA Purified

Description

• Carry out studies involving cell permeability with Thermo Scientific™ Remel™ Phytohaemagglutinin (PHA)
• PHA is a mitogenic agent derived from extracts of Phaseolus vulgaris seeds and has twin properties of causing erythroagglutination and of stimulating progressive lymphocyte mitosis in cell culture
• PHA has proved to be beneficial in the study of the immune response, lymphocyte kinetics, and bone marrow dynamics, and for these purposes it is desirable to use a substance which has more closely reproducible qualities and known potency
• Purified phytohaemagglutinin is a freeze-dried, highly refined protein fraction of selected Phaseolus spp
• seed extract in which the specific mitogenic activity and the mitogenic/ haemagglutinating activity ratio have been increased by a factor of about 100/1 during purification
• Both agglutinating and mitogenic activities of PHA are found to be associated with the protein fractions of crude extracts, which are sufficiently alike in their physico-chemical properties, thus it is difficult to separate them
• Relatively crude extracts selected for their ability to yield good mitotic pictures have proved to be useful in routine lymphocyte culture for chromosome studies
• Ready to use - Prepared reagent Easy to store - Store at 2° to 8°C until use Can be used in routine lymphocyte culture for chromosome studies Purified phytohaemagglutinin contains a freeze-dried, highly refined protein fraction of selected Phaseolus spp
• seed extract
• Each bottle contains 2mg lyophilised phytohaemagglutinin (purified), dried from a small volume of buffered saline without preservative
• The reagent may be reconstituted to any desired volume in sterile saline (if buffered, preferably to a pH between 6.5 and 8) or distilled water, using a sterile disposable hypodermic syringe
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.