Corning™ BioCoat™ Poly-D-Lysine/Laminin Multiwell Plate

Description

• For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone
• Corning BioCoat PDL/LM and PLO/LM Cultureware is suitable for culturing many different types of Peripheral Nervous System (PNS) and Central Nervous System (CNS) networks and is useful for promoting neural cell attachment and differentiation
• Corning BioCoat LM/HFN Cultureware provides an in vitro environment that promotes cell attachment and extensive process formation
• Applications include: Enhancement of neuronal cell attachment to plastic and glass Promotion of neurite outgrowth Culture of glial cells as a feeder layer for neurons Construction of neural cell model systems to study CNS function, development, and diseases Source PDL, synthetic (MW 75-150 kD) PLO, synthetic (MW 30-70 kD) Laminin, EHS mouse tumor Fibronectin, human plasma NOTE: Source material tested for hepatitis B antigen and HIV-1 antibody
• Quality Control PDL/LM and PLO/LM tested for ability to initiate differentiation (neurite outgrowth) of NG-108 rat glioma/mouse neuroblastoma cells LM/HFN tested for receptor agonist induced changes in intracellular calcium using Fluo-3A (a long wavelength fluorescent calcium indicator) in primary rat cortical neuron enriched cultures Tested and found negative for bacteria and fungi Storage 2° to 8°C
• Do not freeze.