Heidolph™ Sphericalplate 5D, 3D Cell Culture Plate

Description

• The Sphericalplate 5D (SP5D) is a 3D cell culture plate for the formation of uniform, standardized, and size-controlled spheroids in high quality and yield
• It also allows you to effortlessly scale up and further translate into diagnostic or clinical applications
• Simultaneously, it increases the reproducibility of subsequent tests, as you always start your experiments with identical initial conditions due to the low size variance of the cultured cell spheroids
• The patented geometry with special coating allows the integration of every single cell into a 3D-agglomerate in a controlled way
• This is how uniformly sized spheroids grow without the formation of necrotic cores
• Design: Patented, three-dimensional geometry with rounded bottoms of the microwells, resulting in scaffold-free, regular spheroid formation while keeping the aggregate in the center
• The microwell geometry prevents hypoxic damage through aggregate size control
• Ease of Use: There are only three main steps in the SOP and it just takes 24 hours for your 9,000 spheroids to be produced
• Step 1) Prepare functional well with medium
• Step 2) Add single cell suspension
• Step 3) Incubate
• Plates are sterilized and have pre-applied anti-adherence coating, making them ready to use out of the box
• No pretreatment or centrifugation steps after seeding your cells
• Patented surface coating to prevent cell-surface signaling during development and eliminates the need for plate pre-treatment
• Special angle of the microwells to allow cells descending in consistent numbers without an additional centrifugation step
• Performance: Spheroid size is controlled by initial seeding density
• No sharp edges at the bottom of the plate to reduce surface-induced, non-physiological differentiation
• Sharp edges at the top of the microwells to leave no cell behind and no unwanted cell growth outside of the microcavities
• Robust non-fouling surface coating featuring low protein-adhesion to allows perfect cell aggregation and prevents surface adhesion
• Aggregate size control through the geometric dimensions of the microwells to avoid hypoxia
• Selection of COC (cyclic olefin copolymers) material to enable optimal real-time imaging with less background noise
• Applications: Standardization of geometric cluster size for clinical applications
• A wide range of cell lines, islet cells, and stem cells have been used on this plate successfully
• 24-Well Plates: Standard 24 circular well plate dimension (12 wells have microwells (3D spheroid production), 12 wells do not have microwells (control))
• Each well has 750 microwells, allowing the plate to produce up to 9,000 spheroids
• Comfortably carry out monolayer comparative studies in the remaining 12 wells of the same plate
• 6-Well Plates: Standard 6 square well plate
• Expected yield of 3,364 spheroids per well, thus a total yield of 20,184 spheroids per plate
• Suitable for scale-up trails and bigger batches.