4070500EB
R&D Systems™ Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer
Manufacturer: R&D Systems™
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Content And Storage
Store at -20° C in a manual defrost freezer. For long term storage, freeze in working aliquots at -80° C. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 4 forimmediate use. TDG protein in storage buffer can survive for up to 24 hours at 37° C with less than 10% loss in activity.
Quantity
500 U
Product Type
Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer
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Description
- TDG is a thermostable thymine DNA glycosylase from Methanobacterium thermoautotrophicum
- The optimal temperature for the enzyme is 65°C
- The enzyme lacks significant AP lyase or endonuclease activity
- One Unit is the amount of enzyme required to cleave 1pmole of an oligonucleotide duplex containing a T/G mismatch in 1 hour at 65°C Only the strand containing the T is cleaved TDG works effectively in heteroduplex analysis to detect C to T transitions
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Content And Storage: Store at -20° C in a manual defrost freezer. For long term storage, freeze in working aliquots at -80° C. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 4 forimmediate use. TDG protein in storage buffer can survive for up to 24 hours at 37° C with less than 10% loss in activity.
Quantity: 500 U
Product Type: Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer
Content And Storage:
Store at -20° C in a manual defrost freezer. For long term storage, freeze in working aliquots at -80° C. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 4 forimmediate use. TDG protein in storage buffer can survive for up to 24 hours at 37° C with less than 10% loss in activity.
Quantity:
500 U
Product Type:
Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer
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