Invitrogen™ Ribonuclease H

Description

• Source: Purified from E
• coli expressing the E
• coli RNase H gene on a plasmid Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease Unit definition: One unit hydrolyzes 1nmol of RNA in 3 H-labeled poly(A)poly(dT) to acid-soluble material in 20 min
• at 37°C Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl 2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3 H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min
• at 37°C Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription Order Info Shipping Conditions: Approved for shipment on wet or dry ice