Invitrogen™ T4 DNA Ligase (1 U/μL)

Description

• T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini
• Single-stranded nucleic acids are not substrates for this enzyme
• Optimizes ligation in 5 minutes Source: Purified from E
• coli lambda lysogen NM989 Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested Unit definition: One unit catalyzes exchange of 1nmol 32 P-labeled pyrophosphate into ATP in 20 min
• at 37°C
• One unit is equal to approximately 300 cohesive-end ligation units Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl 2 , 10mM DTT, 66μM ATP, 3.3μM 32 P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min
• at 37°C ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning Order Info Shipping Conditions: Dry ice