Thermo Scientific Chemicals Deoxyribonuclease I, bovine pancreas, Purified

Description

• Deoxyribonuclease I, is used in RNA preparations from tissue or bacterial cell cultures
• Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase
• It is used in investigating the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis
• This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio
• Some documentation and label information may refer to the legacy brand
• The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals
• General Description • Deoxyribonuclease I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates
• In the presence of Mg2+, this compound cleaves each strand of DNA independently Application • In biomedical research, this compound is used to nick DNA as a first step to integrate labeled bases into DNA • It can be used to eliminate genomic DNA from RNA solutions before RT-PCR • It is also used during isolation and purification of plasma membrane vesicles from Neurospora crassa cell culture • It can decrease viscosity in cell culture lysates, providing better yields in primary cell isolation • It is also commonly used in combination with trypsin for the preparation of single cell suspension from rats • Deoxyribonuclease I, bovine pancreas has been used in some studies in order to compare different methods for reducing RNase contamination in preparations of DNase • This compound can be applied in studies to investigate the impact of the composition of sodium dodecyl sulfate (SDS) preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis