Invitrogen™ TURBO DNA-free™ Kit

Description

• Description Kit contains reagents for efficient, complete digestion of DNA along with removal of enzyme and divalent cations post-digestion Hyperactive TURBO DNase is catalytically superior enzyme compared to wild type DNase I Removes trace quantities of DNA that can interfere with RT-PCR Reagent included to completely remove DNase without phenol treatment or heating Method for genomic DNA removal prior to RT-PCR TURBO DNase is recombinant, engineered form of DNase I that is efficient than wild type DNase I in digesting away trace amounts of unwanted DNA TURBO DNase binds DNA substrates 6-fold more tightly than traditional DNase I, making enzyme tool of choice for clearing residual DNA that can generate a false positive signal in RT-PCR applications TURBO DNase now includes an enhancer that increases effectiveness by two orders of magnitude Efficient DNase and divalent cation removal without organic extraction or precipitation Conventional DNase treatment of RNA samples prior to RT-PCR typically call for inactivation of the DNase by phenol:CHCl 3 extraction or heating followed by precipitation step to concentrate the RNA Phenol:CHCl 3 extractions can be cumbersome and time-consuming Heating the sample to inactivate DNase can lead to chemical degradation of RNA by divalent cations present in DNase buffer TURBO DNA- free Kit circumvents problems using novel DNase Inactivation Reagent For removing the TURBO DNase from reaction, Inactivation Reagent also binds and removes divalent cations from TURBO DNase Reaction Buffer PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription Order Info Shipping Conditions: Dry ice