Invitrogen™ Ambion™ RNase I, cloned, 100 U/μL

Description

• RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate
• RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA
• It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays
• Supplied in one tube of 10,000U (100U/μL) Endonuclease-free, exonuclease-free, protease-free Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min
• at 37°C DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting Order Info Shipping Conditions: Dry ice