Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Description

• Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins
• Proteinase K remains active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g., in the presence of SDS or urea) • In the presence of metal chelating agents (e.g., EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization
• Performance and quality testing Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity
• Unit definition One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate
• Order Info Shipping Condition: Dry Ice