Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL)

Description

• Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus
• The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA
• Endo IV possesses also a 3'≥5' exonuclease activity
• Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions
• Substrates with 3'-recessed ends are preferred substrates for the 3'≥5' exonuclease activity
• The enzyme has no requirement for Mg 2+ and is fully active in the presence of EDTA in moderate concentrations
• Applications Studies of DNA damage and repair Single cell electrophoresis (comet assay) Antitumor drug research DNA structure research SNP analysis