MP Biomedicals™ Hyaluronidase, Streptomyces hyalurolyticus

Description

• Hyaluronidase is the enzyme that degrades hyaluronic acid in mucopolysaccharides to low molecular saccharides
• Hyaluronidase purified from Streptomyeshyalurolyticus is distinct from the known hyaluronidase in its substrate specificity and mechanism of action
• This enzyme is quite specific for hyaluronic acid
• Various types of chondroitin sulfate (A,B,C,D,,E), chondroitin, heparin, keratan sulfate and chitin are not attacked by this enzyme
• This enzyme splits the N-acetylglucosaminic linkages in hyaluronic acid eliminatively , yielding ▿-4,5-unsaturated tetrasaccharides and hexasaccharides as the end-product
• Formula Weight: 55kDa CAS #: 37326-33-3 Synonyms: EC 4.2.2.1; Hyaluronate lyase; Mucinase Physical Appearance : White lyophilized powder Typical : ã 2,000 TRU/mg protein Contaminants : Protease activity: less than 0.05 u/ampule
• One unit is the amount of protease activity which produces 100 ++g of tyrosine from milk casein at 37°C for 60 min
• (pH 6.0) Optimum pH: 6.0 Recommended reaction temperature : 60°C Molecular Weight : 91,000 (gel filtration) Inhibitors : Mn2+, Hg2+, Potassium ferricyanide Unit Definition : One TRU (Turbidity Reducing Unit) is that amount of enzyme which causes 50% of decrease in absorbance at 660 nm in 30 minutes at 60°C.