Promega DNA-Dependent Protein Kinase

Description

• Isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30°C Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl 2 , 1mM DTT, 10% glycerol