Thermo Scientific™ FastDigest SacI

Description

• FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
• The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps
• DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer
• Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
• For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
• Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
• Thermo Scientific FastDigest SacI restriction enzyme recognizes GAGCT^C site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer
• Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI
• Thermo Scientific FastDigest SacI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
• The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps
• See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes
• DNA modifying enzymes, such as Klenow Fragment (Cat
• No
• EP0051), T4 DNA Ligase (Cat
• No
• EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat
• No
• EP0061) all have 100% activity in FastDigest Buffer
• Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
• For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
• Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
• Features • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer
• For applications that require product analysis by fluorescence excitation (e.g
• concentration measurements in UV light) the colorless FastDigest Buffer is recommended
• For methylation sensitivity, refer to product specifications.