7075671

CCF4-AM

CCF4-AM

Manufacturer: Fischer Scientific

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Cell Permeability

Cell-Permeant

Detection Method

Fluorescence

Substrate

CCF4-AM

Substrate Type

Beta-Lactamase Substrate

Shipping Condition

Room Temperature

Content And Storage

Store in freezer (-5 to -30°C).

Form

Solid

Substrate Properties

Chemical Substrate

For Use With (Equipment)

Flow Cytometer, Microplate Reader

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Description

  • CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells
  • Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol
  • CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein
  • This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer™ technology.

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Fluorescence

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Beta-Lactamase Substrate

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Room Temperature

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Store in freezer (-5 to -30°C).

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Solid

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