PV4812

Fluorescein-MAP2K1

Manufacturer: LIFE TECHNOLOGIES

Select a Size

Pack Size SKU Availability Price
Each of 1 PV4812-Each-of-1 In Stock ₹ 87,784.56

PV4812 - Each of 1

₹ 87,784.56

In Stock

Quantity

1

Base Price: ₹ 87,784.56

GST (18%): ₹ 15,801.221

Total Price: ₹ 1,03,585.781

Assay Entry

Biochemical terbium phospho-specific activity

Detection Method

Fluorescence

Substrate

MAP2K1

Substrate Type

Kinase Substrate

Gene ID (Entrez)

10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885

Molecular Weight (g/mol)

50000 Da

Target Entry

MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3

Content And Storage

Fluorescein-MAP2K1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1mM DTT. Store at -80°C.

Quantity

20 nmol

Substrate Properties

Protein-Based Substrate

Target Enzyme

Serine/Threonine Kinases

Label or Dye

FITC (Fluorescein)

Shipping Condition

Dry Ice

Validated Kinases

BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11

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Description

  • Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members
  • For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening applications.

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Assay Entry:
Biochemical terbium phospho-specific activity

Detection Method:
Fluorescence

Substrate:
MAP2K1

Substrate Type:
Kinase Substrate

Gene ID (Entrez):
10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885

Molecular Weight (g/mol):
50000 Da

Target Entry:
MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3

Content And Storage:
Fluorescein-MAP2K1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1mM DTT. Store at -80°C.

Quantity:
20 nmol

Substrate Properties:
Protein-Based Substrate

Target Enzyme:
Serine/Threonine Kinases

Label or Dye:
FITC (Fluorescein)

Shipping Condition:
Dry Ice

Validated Kinases:
BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11

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PV4815

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Assay Entry:
__

Detection Method:
__

Substrate:
__

Substrate Type:
__

Gene ID (Entrez):
__

Molecular Weight (g/mol):
__

Target Entry:
__

Content And Storage:
Terbium-labeled peIF2a (pThr46) antibody in HEPES buffered saline. Store at -20°C.

Quantity:
25 μg

Substrate Properties:
__

Target Enzyme:
__

Label or Dye:
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Shipping Condition:
Dry Ice

Validated Kinases:
__

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Assay Entry:
__

Detection Method:
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Substrate:
__

Substrate Type:
__

Gene ID (Entrez):
__

Molecular Weight (g/mol):
__

Target Entry:
__

Content And Storage:
Terbium-labeled peIF2a (pThr46) antibody in HEPES buffered saline. Store at -20°C.

Quantity:
1 mg

Substrate Properties:
__

Target Enzyme:
__

Label or Dye:
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Shipping Condition:
Dry Ice

Validated Kinases:
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Assay Entry:
__

Detection Method:
__

Substrate:
__

Substrate Type:
__

Gene ID (Entrez):
__

Molecular Weight (g/mol):
__

Target Entry:
__

Content And Storage:
Terbium-labeled pMAP2K1 (pSer217/221) antibody in HEPES buffered saline. Store at -20°C.

Quantity:
25 μg

Substrate Properties:
__

Target Enzyme:
__

Label or Dye:
__

Shipping Condition:
Dry Ice

Validated Kinases:
__