Thermo Scientific™ ALA Disk

Description

• Rapidly differentiate Haemophilus species on the basis of the detection of porphyrin synthesis using Thermo Scientific™ Remel™ ALA Disk
• In 1974, Kilian described a rapid method for identification of X-factor independent Haemophilus spp
• based on the ability to synthesize heme precursors from ALA 1
• Lund and Blazevic determined the porphyrin test is more rapid and accurate for identification of X-factor-independent strains of Haemophilus (e.g
• H
• parainfluenzae and H
• parahaemolyticus ) than the satellite test 2
• Members of the genus Haemophilus are small non-motile Gram-negative bacilli traditionally defined by a requirement for X and/or V factor 3
• X factor is derived from hemoglobin and V factor through the satellite relationship with Staphylococcus aureus
• Rapidly differentiate Haemophilus species on the basis of their detection of porphyrin synthesis using ALA Disk
• Ready to use: Reagent-impregnated disk Rapid test: Results are obtained within a few hours
• Accurate test: For detection of porphyrins which are only excreted by X-factor-independent Haemophilus spp
• Easy to identify: A positive result showcases bright yellow fluorescence under long wave ultraviolet light
• After incubation the disk is exposed to long wave ultraviolet light in a dark room for any indication of fluorescence
• Biberstein et al
• studied the action of Haemophilus on δ-aminolevulinic acid (ALA) and reported a perfect correlation between X-factor-independence and the ability to convert ALA to porphyrins 4
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.