Thermo Scientific™ PathoDX™ Strep Grouping Kit

Description

• Use Thermo Scientific™ Remel™ PathoDx™ Strep Grouping kit to perform a latex agglutination test designed to identify beta-hemolytic streptococci of Lancefield Groups A, B, C, F and G from primary culture plates
• The PathoDx procedure utilizes a latex agglutination method in conjunction with a nitrous acid extraction procedure 1,2
• The IgG coupled to the latex is highly specific for a given streptococcal group antigen
• This method offers significant advantages over other streptococcal grouping procedures in terms of rapidity, simplicity, and convenience
• The streptococcal group carbohydrates of streptococcal groups A, B, C, F and G are complex antigens with a rhamnose oligosaccharide and differing side chains, consisting primarily of glucosamine, either acetylated or non-acetylated
• In the PathoDx procedure, specific antibody on latex particles reacts with, and agglutinates, streptococcal group antigen extracted from the bacterial cell wall
• The test is intended for use with streptococcal colonies that are beta-hemolytic on sheep blood agar or for use with pure streptococcal isolates in broth cultures
• The reagents are designed to give a positive (2+ or greater) agglutination with one to two 18 to 24 hour colonies for most beta-hemolytic streptococcal isolates
• Ready-to-use – Prepared reagents Easy to interpret – The PathoDx Strep Grouping kit is designed to give a 2+ or greater agglutination reaction with the extract of one or two colonies of an 18 to 24 hour culture of streptococci of Lancefield groups A, B, C and G (large colony variety) in 60 seconds for most streptococcal isolates
• Minute colony Group F and small colony strains of other groups require many more colonies (heavy sweep) to give a positive agglutination reaction
• A negative test produces a uniform milky appearance with no agglutination after 60 seconds
• The Grouping Latexes are presented as five dropper bottles, one specific for each of the groups A, B, C, F and G, each containing 3.0mL of a suspension of synthetic blue latex particles coated with rabbit antibody IgG-sensitized with 0.098% sodium azide and 0.05% ProClin 300 ® as preservatives
• The PathoDx Strep Grouping kit may also be used with beta-hemolytic streptococci grown in broth in pure culture
• The kit contents the following reagents: Strep A Grouping Latex (R62030) - 1 dropper bottle (red cap) Strep B Grouping Latex (R62031) - 1 dropper bottle (pink cap) Strep C Grouping Latex (R62032) 1 dropper bottle (orange cap) Strep F Grouping Latex (R62034) - 1 dropper bottle (blue cap) Strep G Grouping Latex (R62035) - 1 dropper bottle (purple cap) Positive Control (R24042) - 1 dropper bottle (yellow cap) Reagent 1 (R62050) - 1 dropper bottle (red cap) Reagent 2 (R62055) - 1 dropper bottle (blue cap) Reagent 3 (R62060) - 1 dropper bottle (green cap) Stirrers - 1 bag Disposable slides (R62070)- 1 pack Not all products are available for sale in all territories
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.