Thermo Scientific™ Sabouraud Dextrose Agar (Dehydrated)

Description

• Identify dermatophytes and other fungi and yeasts in clinical specimens and environmental samples with the acidic Thermo Scientific™ Oxoid™ Sabouraud Dextrose Agar
• The low pH supports the growth of fungi while inhibiting bacterial growth in mixed samples
• Fungi maintain their typical cultural appearance and, thus, may be readily identified according to standard macroscopic characters
• Cultivate and differentiate fungi with Oxoid Sabouraud Dextrose Agar, a modification of Sabouraud agar (Carlier 1 )
• The medium is often used with antibiotics for the isolation of pathogenic fungi from material containing large numbers of other fungi or bacteria
• Georg et al
• 2 aseptically added 0.5g cycloheximide, 20,000 units penicillin and 40,000 units streptomycin to each litre of autoclaved, cooled medium
• Cryptococcus neoformans , Aspergillus fumigatus and Allescheria boydii are sensitive to cycloheximide; Actinomyces bovis and Nocardia asteroides are sensitive to penicillin and streptomycin
• Alternatively, one may add 0.4g chloramphenicol and 0.05g cycloheximide to each liter of reconstituted medium before autoclaving (Ajello 3 )
• The same microorganisms are sensitive to this new combination (see Dermasel Selective Supplement SR0075E)
• Williams Smith & Jones 4 employed Oxoid Sabouraud Dextrose Agar, containing 20,000 units penicillin and 0.04g neomycin per liter, for the count of yeasts in the alimentary tract of the pig
• Hantschke 5 used colistin, novobiocin and cycloheximide to isolate Candida albicans
• Dolan 6 used gentamicin, chloramphenicol and cycloheximide for the selective isolation of pathogenic fungi
• Oxoid Sabouraud Dextrose Agar may also be used as the basis of a Pagano-Levin medium 7 for the isolation of Candida albicans
• 0.1g of triphenyltetrazolium chloride (as a filter sterilised solution) is added to each liter of autoclaved molten medium cooled to 55°C
• The medium is usually made inhibitory to most non-pathogenic fungi and bacteria by the addition of antibiotics, as above
• After incubation for three days at 25°C, Candida albicans colonies are unpigmented or pale pink while other Candida species and other fungi form deeper pink or red colonies
• The test is adequate for screening purposes, but other diagnostic criteria should also be utilized for the identification of Candida albicans 8 The combination of cycloheximide and chloramphenicol inhibits many pathogenic fungi 2
• However, the mycelial phase of Histoplasma capsulatum , Paracoccidioides brasiliensis , Sporothrix schoenckii and Blastomyces dermatitidis is not inhibited by these antibiotics when incubated at 25-30°C 9
• Please check relevant health and safety documentation before working with cyclohexamide
• Some of the pathogenic fungi may produce infective spores which are easily dispersed into the laboratory
• Such organisms should be examined only within a protective cabinet
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