Thermo Scientific™ Bismuth Sulphite Agar (Modified Wilson & Blair Medium) (Dehydrated)

Description

• Isolate Salmonella typhi and other salmonllae from pathological material, sewage water supplies, food and other products with Thermo Scientific™ Oxoid™ Bismuth Sulphite Agar (Modified Wilson & Blair Medium) (Dehydrated)
• Bismuth Sulphite Agar is a modification of the original Wilson and Blair 1 Medium and is particularly useful for the isolation of lactose-fermenting salmonellae
• The presence of bismuth sulphite and brilliant green make this medium highly selective for salmonellae
• Salmonella infection (salmonellosis) is a common bacterial disease that affects the intestinal tract
• Salmonella bacteria typically live in animal and human intestines and are shed through feces
• Humans become infected most frequently through contaminated water or food
• Bismuth Sulphite Agar is a selective medium for the isolation and preliminary identification of Salmonella typhi and other salmonellae from pathological material, sewage, water supplies, food and other products suspected of containing these pathogens
• Selective: bismuth sulphite together with brilliant green acts as a selective agent making the medium selective for salmonellae while suppressing the growth of coliforms
• Easy-to-read: Salmonellae typically grow as black colonies with a surrounding metallic sheen resulting from hydrogen sulphide production and reduction of sulphite to black ferric sulphide
• Atypical colonies may appear if the medium is heavily inoculated with organic matter
• Such a situation may be prevented by suspending the sample in sterile saline and using the supernatant for inoculation
• The freshly prepared medium has a strong inhibitory action 2 and is suitable for heavily contaminated samples
• Storing the poured plates at 4°C for 3 days causes the medium to change color to green, making it less selective with small numbers of salmonellae being recovered 3
• However, for Salmonella typhi recovery the latter technique is not recommended 4
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.