Thermo Scientific™ DNASE Agar (Dehydrated)

Description

• Detect microbial deoxyribonuclease enzymes, particularly from staphylococci, with Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated)
• A DNase (deoxyribonuclease) is any enzyme that catalyzes the cleavage of phosphodiester linkages of DNA leading to its degradation
• The organisms producing DNase enzymes in sufficient quantities to hydrolyze DNA will show clear zones around the colonies
• The medium is mainly used in the identification of staphylococci but may also be used for the detection of DNase activity in other organisms
• Weckman & Catlin 1 suggested that DNase activity could be used to identify pathogenic staphylococci after they had established a close correlation with coagulase production
• Jeffries et al
• 2 incorporated DNA in the agar medium to provide a simple method of detecting DNase activity
• Easily differentiates Staphylococcus aureus from Staphylococcus epidermidis and other Staphylococcus species due to the abundance of DNase production in Staphylococcus aureus
• 2,3,4 Helps in the differentiation and identification of non-pigmented Serratia marcescens (positive reaction) from Klebsiella-Enterobacter (negative reaction) 5
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.