Thermo Scientific™ M17 Broth (Dehydrated)

Description

• Cultivate lactic streptococci and their bacteriophages with Thermo Scientific™ Oxoid™ M17 Broth (Dehydrated)
• The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine 1 for bacteriophage assays
• Lactic streptococci are nutritionally fastidious and require complex media for optimal growth 2,3
• Their homofermentative acid-producing nature require that the medium is well buffered so that the culture pH is maintained above 5.7 during active growth
• This maintenance of the pH is important as lower pH can results in injury and reduced recovery of lactic streptococci
• M17 Broth contains di-sodium glycerophoshate which has sufficient buffering capacity to maintain the pH above 5.7 of actively growing cultures even after 24 hours at 30°C
• This buffering agent also allows the addition of calcium without a precipitation complex being formed
• The calcium-containing medium is used for the assay of bacteriophages of lactic streptococci 1
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.