Thermo Scientific™ MacConkey Sorbitol Agar (Dehydrated)

Description

• Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with Thermo Scientific™ Remel™ MacConkey Sorbitol Agar (Dehydrated)
• This serotype of E
• coli produces a verotoxin and causes haemorrhagic colitis outbreaks 1 and is indistinguishable on the routine isolation media from those of non-VTEC E.coli
• In 1986, March and Ratnam introduced MacConkeys Agar with sorbitol for the isolation of this serotype as unlike other serotypes VTEC does not ferment sorbitol 2
• Haldane et al
• since then implemented the use of this medium as the primary isolation medium 3
• Escherichia coli O157 is recognized as a cause of haemorrhagic colitis, an illness characterized by bloody diarrhoea and severe abdominal pain, and haemolytic uraemic syndrome (HUS) and as such, is a significant human pathogen
• Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with MacConkey Sorbitol Agar
• Ease of Differentiation: Medium contains D-sorbitol; sorbitol negative strain forms colorless colonies while other E
• coli strains ferment sorbitol and form pink colonies
• Recommended as a simple, inexpensive, rapid and reliable means of screening for E
• coli O157 This medium is used for qualitative procedures
• This medium contains gelatin, casein and meat peptones that provides nitrogen, amino acids and peptides essential for bacterial growth
• Bile salts and crystal violet are selective agents which inhibit gram-positive organisms
• Lactose is substituted with D-sorbitol
• Vertoxin producing strains of E.coli ferment lactose but not sorbitol and form colorless colonies
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.