Thermo Scientific™ Brilliance™ Bacillus Cereus Agar Base (Dehydrated)

Description

• Isolate and differentiate Bacillus cereus from food samples with chromogenic Thermo Scientific™ Brilliance™ Bacillus Cereus Agar Base (Dehydrated)
• Brilliance Bacillus cereus Agar incorporates the chromogenic substrate 5-bromo-4-chloro-3-indolyl-ß-glucopyranoside, which is cleaved by the enzyme ß-glucosidase present in Bacillus cereus resulting in the formation of blue/green colonies
• Combination of Polymixin B and Trimethoprim inhibit most Gram-positive bacteria and Gram-negative bacteria other than Bacillus cereus respectively
• Bacillus cereus , a Gram-positive, aerobic, spore-forming rod-shaped bacterium, is widely distributed in nature
• It is readily isolated from soil, dust, cereal crops, vegetation, animal hair, fresh water and sediments
• Therefore, it is not surprising to find the organism associated with virtually every raw agricultural commodity
• The role of Bacillus cereus in food poisoning, particularly from the consumption of contaminated rice, is now well documented 1,2,3
• The organism has also been implicated in eye infections 4,5 and a wide range of other conditions, including abscess formation, meningitis, septicemia, and wound infection
• Easy-to-read : cleavage of the chromogenic substrate the enzyme ß-glucosidase present in Bacillus cereus results in the formation of blue/green colonies
• Selective : a combination of Polymixin B and Trimethoprim make the medium selective for Bacillus cereus
• Brilliance Bacillus cereus Agar incorporates the chromogenic substrate 5-bromo-4-chloro-3-indolyl-ß-glucopyranoside, which is cleaved by the enzyme ß-glucosidase present in Bacillus cereus resulting in the formation of blue/green colonies
• Polymixin B inhibits most Gram-negative organisms and some Gram-positive organisms including some Bacillus other than Bacillus cereus
• Trimethoprim, which is also added to the medium, blocks folic acid synthesis necessary for DNA production and is active against many Gram-positive bacteria including Staphylococcus aureus , Enterococcus spp
• and some non-cereus Bacillus species
• The combination of these two antibiotics has been shown to be more effective than the use of polymyxin B alone 3
• Because Bacillus thuringiensis is biochemically identical to Bacillus cereus, it will also grow as blue/green colonies on this medium
• Bacillus thuringiensis is known primarily as an insect pathogen, but it has also been reported to have been linked to some human gastroenteritis outbreaks 4
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