Invitrogen™ One Shot™ LB Agar Plates with or without Antibiotics

Description

• One Shot LB Agar Plates are pre-poured plates containing Luria-Bertani (LB) agar medium that are available with or without antibiotics
• LB agar is a solid bacterial growth medium used in molecular biology studies for the cultivation and maintenance of Escherichia coli strains
• Autoclaved glass beads are included to aid in plating
• LB agar is a popular, nutrient-rich medium made from casein peptone, yeast extract, sodium chloride, and agar
• Casein peptone or tryptone is an enzymatic hydrolysate of casein
• It supplies nitrogen, carbon, and amino acids
• Yeast extract provides trace elements, vitamins (including B group vitamins), amino acids, and peptides
• Sodium chloride supplies sodium cations for membrane transport and maintains osmotic balance
• Agar is a solidifying agent of the growth medium
• One Shot LB Agar Plate composition is based on the Jeffrey H
• Miller formulation and contains twice as much sodium chloride as the Lennox formulation
• The inclusion of autoclaved glass beads allows for simple plate inoculation without the need for additional loops or spreaders
• As E
• coli is unable to digest agar but can absorb nutrition from the LB medium, single colonies can be easily isolated and selected
• On plates with a selective antibiotic only transformed E
• coli with resistance can grow
• One Shot LB Agar Plate formulation 10 g/L casein peptone, 5 g/L yeast extract, 10 g/L NaCl, 15 g/L agar Benefits of One Shot LB Agar Plates Read-to-use format conveniently packaged in two sleeves of 10 plates Long-shelf life: 3.5 months without antibiotic and 3 months with antibiotic Autoclaved glass plating beads provided for easy cell spreading One Shot LB Agar Plates are a good choice for use with our Gateway, TOPO, and Gibson Assembly cloning kits and One Shot competent cells to provide faster, reproducible results to support your downstream applications
• Primary applications Selecting transformed coli colonies using antibiotics Streaking and isolating bacteria cells on LB agar plate Bacteriophage plaque assay using a lawn of bacteria Discover innovative products for your cloning workflow We offer a wide selection of ready-to-use bacterial growth media formulations, such as LB (Lennox), Terrific Broth, and S.O.C recovery medium
• With our selection guide you can explore our One Shot competent cell strains and discover a wide range of cloning solutions for routine and specialty applications, along with our gene synthesis services.