Thermo Scientific™ Levine EMB Agar

Description

• Carry out selective and differential isolation of Gram-negative enteric bacteria from clinical specimens with Thermo Scientific™ Remel™ Levine EMB Agar
• The EMB Agar was originally developed by Holt-Harris and Teague 1
• The combination of dyes in the medium and the incorporation of lactose and sucrose provided a differential plating medium for the Enterobacteriaceae
• The Levine modification of EMB Agar does not contain sucrose, and the lactose concentration is increased 2
• Levine claimed that this formulation provided better differentiation between Escherichia coli and Enterobacter species
• Levine EMB Agar is a solid medium recommended for use in qualitative procedures for selective and differential isolation of Gram-negative enteric bacilli from clinical specimens
• Easy-to-read : due to Eosin–Y and methylene blue indicators Escherichia coli (purple colonies with a green metallic sheen) are easy to differentiate from Enterobacteria aerogenes (purple mucoid colonies)
• Non-lactose fermenters, such as Shigella and Salmonella form mucoid, pink brown colonies in less acidic conditions
• Selective: the presence of Eosin and methylene blue makes the medium slightly selective and inhibit certain Gram-positive bacteria
• Ready-to-use : convenience of prepared media
• Eosin and methylene blue dyes provide for differentiation of enteric Gram-negative bacilli based on the fermentation of lactose and the absorption of the dyes by the bacterial colonies
• Eosin blue combines with methylene blue indicator to produce a color change when lactose is fermented
• As the Levine modification does not contain sucrose and therefore, slow lactose-fermenters may mimic the appearance of enteric pathogens
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand