Thermo Scientific™ Mannitol Salt Agar

Description

• Selectively and differentially isolate and identify staphylococci from clinical and non-clinical samples using Thermo Scientific™ Remel™ Mannitol Salt Agar
• This phenol red mannitol agar contains 7.5% sodium chloride as a selective agent as reported by Koch in 1942 1
• Most strains of coagulase-positive staphylococci grow on this agar, producing yellow zones because of mannitol fermentation whereas coagulase-negative staphylococci strains produce small colonies with no color change in the surrounding medium
• Staphylococci are widespread in nature, although they are mainly found on the skin, skin glands and mucous membranes of mammals and birds
• The coagulase-positive species i.e., Staphylococcus aureus is well documented as a human opportunistic pathogen
• The ability to clot plasma continues to be the most widely used and accepted criterion for the identification of pathogenic staphylococci associated with acute infections 2
• Mannitol Salt Agar is a selective and differential solid media for isolating staphylococci bacteria by using their capability to ferment mannitol by the production of coagulase enzyme
• Easy to differentiate : Differentiates between coagulase-positive and coagulase-negative staphylococci
• Selective : Sodium chloride in the concentration of 7.5% inhibits many bacteria other than staphylococci
• This medium contains peptones and beef extract, which supply nitrogen, vitamins, minerals, and amino acids essential for growth
• The 7.5% concentration of sodium chloride results in the partial or complete inhibition of bacterial organisms other than staphylococci
• Sodium chloride also supplies essential electrolytes for transport and osmotic balance
• Mannitol is the fermentable carbohydrate, fermentation of which leads to acid production, detected by phenol red indicator, and aids in the differentiation of staphylococcal species
• Coagulase positive staphylococci produce yellow colonies and a surrounding yellow medium while coagulase negative staphylococci produce red colonies and no color change of the phenol red indicator
• Agar is the solidifying agent
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.