Thermo Scientific™ Indole Broth (4mL)

Description

• Determine the indole production by members of the Enterobacteriaceae using Thermo Scientific™ Remel™ Indole Broth
• Indole production is an important characteristic in the identification of many species of bacteria, specifically enteric gram-negative rods
• Indole Broth is recommended by the American Public Health Association (APHA) and in the Identification of Enterobacteriaceae for use in the differentiation of enteric gram-negative rods 1,2
• Microorganisms that possess the enzyme tryptophanase have the ability to hydrolyze and deaminate tryptophan with the production of indole, pyruvic acid and ammonia 3
• Indole that splits from the tryptophan molecule on addition of the reagent ρ-dimethylaminobenzaldehyde results in a distinct red color that can be easily detected 4
• Ready to use - Liquid media Recommended by APHA in the Identification of Enterobacteriaceae for use in the differentiation of enteric gram-negative rods 1,2
• Easy to interpret - Positive test - Pink to red colored ring at the interface of the broth and the reagent Negative test - No color development Indole Broth contains casein peptone that supplies essential growth factors, trace elements, and is the source of tryptophan in this medium.
• Sodium chloride maintains osmotic equilibrium
• Certain gram-negative rods release indole after hydrolyzing tryptophan
• After addition of dimethylaminobenzaldehyde, it combines with the indole and produces a distinct red color.