Thermo Scientific™ Regan Lowe Semi-Solid Transport

Description

• Selectively isolate Bordetella pertussis and Bordetella parapertussis with Thermo Scientific™ Remel™ Regan-Lowe Semi-Solid Transport Medium
• Regan and Lowe demonstrated that charcoal agar supplemented with 40μg/mL cephalexin and 10% defibrinated horse blood was an excellent enrichment and transport medium 1
• Hoppe reported that horse blood was superior to sheep or human blood for recovery of Bordetella spp
• and incubation in ambient air results in more luxuriant growth than incubation in 5-10% CO 2 2
• B
• pertussis and B
• parapertussis that cause whooping cough, are amongst the most fastidious bacteria known and require special culturing techniques
• Regan-Lowe transport medium is semi-solid in nature and is used for transportation and selective isolation of Bordetella pertussis and Bordetella parapertussis
• The medium contains Cephalaxin which inhibits penicillin-resistant staphylococci, and some coliforms, as well as strains of Haemophilus influenzae and allows selective isolation of Bordetella spp
• from respiratory specimens
• Ready-to-use – Prepared media in 15mm x 103mm tubes (5mL) Selective – Cephalexin and Amphotericin B antibiotics inhibit penicillin resistant staphylococci, coliforms, Haemophilus influenzae ; yeasts and fungi respectively and provides selective isolation of Bordetella spp
• Regan-Lowe Semi-Solid Transport Medium contains charcoal agar base with 10% lysed horse blood which provides an enriched medium
• Nicotinic acid is added as an essential nutrient for Bordetella
• Horse blood acts as a growth factor for B
• pertussis and as a neutralizer of inhibiting substances 2
• Cephalexin inhibits penicillin resistant staphylococci, coliforms and, Haemophilus influenzae
• Amphotericin B inhibits growth of yeasts and fungi
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.