Thermo Scientific™ SIM Medium

Description

• Differentiate enteric gram-negative bacilli based on sulfide production, indole formation, and motility using Thermo Scientific™ Remel™ SIM Medium (Sulfide, Indole, Motility)
• Sulkin and Willet reported that H 2 S detection is an important parameter to differentiate Salmonella and Shigella from other members of the family Enterobacteriaceae 1
• Blazevic used motility-indole medium to differentiate Klebsiella from Enterobacter and Serratia 2
• Green et al., using smaller quantities of SIM Medium, demonstrated motility results could be interpreted after incubation periods shorter than 18-24 hours 3
• Sulfide, indole and motility have been combined in one tube for easy use
• Indole is detected by adding Kovac's Reagent or Ehrlich's Reagent and it combines with para-dimethylaminobenzaldehyde to produce a red complex
• Addition of agar forms a semisolid medium which is ideal for the detection of motility
• Ready to use – Convenience of prepared medium Easy to interpret Hydrogen Sulfide (S) Positive test – Blackening along the line of inoculation Negative test – No blackening of the medium After addition of Indole (I) reagent Positive test – Red color development in the upper portion of the medium Negative test – No red color development Motility (M) Positive test – Diffuse growth outward from the stab line or turbidity throughout the medium Negative test – Growth only along the line of inoculation This medium contains casein and meat peptones that supply nitrogenous compounds and amino acids necessary for growth of enteric gram-negative bacilli
• Sodium thiosulfate is a sulfur source
• Ferric ammonium citrate is an indicator which reacts with H 2 S produced by sulfate-reducing bacteria to form a black precipitate, ferrous sulfide
• Organisms that possess the enzyme tryptophanase degrades tryptophan to produce indole which is detected by the addition of Kovac's or Ehrlichs's reagent
• The addition of agar results in a semisolid medium suitable for the detection of motility
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.