Thermo Scientific™ L-J Gruft

Description

• Selectively isolate and cultivate Mycobacterium spp
• using Thermo Scientific™ Remel™ Lowenstein-Jensen (L-J) Medium, L-J Gruft, and L-J Mycobactocel
• Medium consisting of congo red and malachite green dyes was formulated by Lowenstein to cultivate mycobacteria 1
• This medium was modified by Jensen by replacing congo red with citrate and phosphate contents and increasing the malachite green concentration 2
• L-J Medium used for recovery of pathogenic Mycobacterium spp
• was found to be superior to other media tested, including ATS (American Trudeau Society) Medium 3,4
• Selectively isolate and cultivate Mycobacterium spp
• using Lowenstein-Jensen (L-J) Medium, L-J Gruft, and L-J Mycobactocel
• Selective – Antibiotics in the medium make the medium selective for Mycobacterium while inhibiting contaminating bacteria
• Ready to use – Convenience of prepared media
• L-J Medium is a glycerated egg-potato medium
• The glycerol and egg mixture provide fatty acids and protein required for the metabolism of mycobacteria
• L-J Medium was modified by Gruft by adding ribonucleic acid as a growth stimulant and penicillin and nalidixic acid as selective inhibitors of contaminating bacteria 5
• Ribonucleic acid increases the percentage of positive cultures over the standard L-J Medium
• The selectivity of L-J Medium was increased by incorporating cycloheximide, lincomycin, and nalidixic acid and was formulated by Petran and Vera 6 .