Selectively isolate Neisseria gonorrhoeae and Neisseria meningitidis with Thermo Scientific™ Remel JEMBEC System with Thayer Martin Agar Improved (M-L) w/anisomycin, colistin, vancomycin and trimethoprim
Johnston introduced a medium to grow N
gonorrhoeae in 24 hours 1 and this was the advent of GC Agar Base
Various studies have demonstrated improved recovery for pathogenic Neisseria spp
using selective media 2-4
Martin and Lewis recommended the use of anisomycin, which is a more stable antifungal agent, to replace nystatin and increase the shelf life of the media 5
Thayer and Martin formulated a selective medium to isolate N
gonorrhoeae and N
meningitidis containing antibiotics capable of suppressing the commensal microbial flora present in the anal canal, vagina, pharynx, and other sites 6
The antibiotics used initially, ristocetin and polymyxin B, were replaced with vancomycin, colistimethate, and nystatin 7
The medium contains an increased concentration of dextrose which allows better growth of gonococci
Ready-to-use – Convenience of prepared medium
Selective media – Anisomycin suppresses Candida albicans
Colistin inhibits most gram-negative microorganisms, including Pseudomonas spp
Vancomycin inhibits gram-positive cocci
Trimethoprim lactate suppresses swarming of Proteus spp
Thayer and Martin Agar (Improved) contains hemoglobin, which provides the X factor (hemin), and GCHI Enrichment that provides the V factor, vitamins, amino acids, coenzymes, and dextrose
The tablet provided in the JEMBEC™ and Pill Pocket systems comes in contact with the water vapor present in the medium and liberates carbon dioxide into the enclosed pack
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.