Overnight Express Autoinduction System 1 - Novagen

Description

• General description: The Overnight Express™ Autoinduction System allows the induction of protein expression without monitoring cell density and without conventional induction with isopropyl β-D-1-thiogalactopyranoside (IPTG). This system is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones. The Overnight Express Autoinduction System together with the Novagen RoboPop Solubility Screening Kit and RoboPop Purification Kits allows rapid optimization of the host-vector combinations, expression conditions, and purification parameters for high-throughput production of proteins for structural or functional analysis.
• Application: Overnight Express™ Autoinduction System 1 - Novagen has been used for the purification of 15N-human prion proteins (HuPrPs). It is suitable for most routine recombinant protein expression applications.
• Features and Benefits: No need to monitor cell growth or add IPTGHigh cell densities and protein expression levelsIncreased soluble protein yieldInduction of numerous expression clones simultaneouslyUsable with any conventional glucose-free bacterial growth mediumIdeal for pET Expression System or other IPTG-inducible bacterial systemsCompatible with cultures grown in flasks, culture tubes, and deep-well platesMinimal sample handling
• Components: The Overnight Express™ Autoinduction System 1 kit contains three concentrated sterile solutions:OnEx Solution 1 A blend of carbon sources optimized for tightly regulated uninduced growth to high cell density, followed by high-level inductionOnEx Solution 2 A concentrated buffer and nitrogen-source bend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesisOnEx Solution 3 Provides magnesium for maximal cell density
• Warning: Toxicity: Standard Handling (A)
• Other Notes: Grabski, A., et al. 2003. inNovations 17, 3.
• Legal Information: Express is a trademark of Merck KGaA, Darmstadt, Germany