Description

• General description: RNase A is a single chain polypeptide containing 124 amino acids, linked by four disulfide bridges.[1] In contrast to RNase B, RNase A is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.
• Application: RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.RNase A is also used in RNA sequence analysis and protection assays.RNase A has been used as a tool for computer-aided drug design.RNase A supports the analysis of RNA sequences.RNase A hydrolyze RNA contained in protein samples.Purification of DNA is supported by RNase A.
• Features and Benefits: Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
• Analysis Note: Protein determined by E.
• Other Notes: A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 μg/mL.Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.