CRISPR GUS GAPDH Reporter Control for Monocots

Description

• General description: All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS ReporterCRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter. Arabidopsis seedlings were germinated in 6 well tissue culture plates. The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product
• Application: To verify successful integration of T-DNA in plant genome GUS receptor wheat gGAPDH control for monocots for Agrobacterium mediated transformation
• Features and Benefits: Low cost, genome editing option compared to other methods.Easy to useOnline orderingReady to ship in 2 days
• Components: 1 vial containing 50ul of 20ng/ul plasmid DNAKeep reagent tubes closed when not in use. Practice aseptic lab technique to avoid DNase contamination.
• Principle: CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
• Other Notes: For ordering any of our custom CRISPR plant products please visit: CUSTOM ORDERING FORM
• Legal Information: CRISPR Use License Agreement